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LEF1 is not induced by CHIR99021 in FLCN-deficient cells. (A) Immunofluorescent images of control and FLCN knockout MRC-5 (clone 5) cells treated with DMSO or CHIR99021 (3 μM, 48 h). Blue is DAPI and green is LEF1 staining, 60× magnification. The data are representative of three independent experiments. (B) Western blot of control and FLCN-deficient MRC-5 cells (clone 5) treated with control or CHIR (3 μM, 48 h). β-actin is a loading control.
