Figure 2.

Overexpression or knockdown of B2R enhances or inhibits, respectively, the proliferation, migration, and invasion of CC cells. (A) SiHa and HeLa cells were transfected with oe-B2R, sh-B2R, or an empty lentiviral vector and analyzed with western blotting and RT-qPCR. (B) Plate colony formation assays were used to assess the proliferation activity of the established cells. (C and D) Transwell assays were used to assess cell migration and invasion by cell numbers. (E and F) Representative images and quantification of the wound closure capacities of the established cells. All experiments were performed in triplicate. Data are presented as the mean ± SD. *P<0.05 and **P<0.01. B2R, bradykinin B2 receptor; CC, cervical cancer.