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. 2019 Oct 22;42(6):2644–2654. doi: 10.3892/or.2019.7382

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Copyright: © Ge et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Effect of Smad3 overexpression on the expression of CHOP and caspase-3. (A) The mRNA expression level of Smad3 in Smad3-overexpressed NS-1 cells was examined by RT-PCR. Lane 1, pcDNA3 empty plasmid group; lane 2, Smad3-overexpression group. The graph represents the fold change in mRNA expression normalized to GAPDH. (B) The protein expression of Smad3, p-Smad3, caspase-3, cleaved caspase-3 and CHOP protein expression was assessed by western blotting. The graph reveals the fold change in protein expression normalized to β-tubulin from three independent experiments. *P<0.05, **P<0.01, compared with the pcDNA3 empty plasmid group. Lane 1, pcDNA3 empty plasmid group; lane 2, Smad3-overexpression group.