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The LAMP primers targeting the N-gene (set F) designed and validated in this study.
| Oligo sequence (5' to 3') | Position | Oligo name | Final concentration in 25 μl LAMP reaction |
|---|---|---|---|
| TGTTAGCCTCCATACTAGCA | 497 | N_F3_f | 0.2 μM |
| TGTCAATGTCGCAGATCATT | 775 | N_B3_f | 0.2 μM |
| TGTCAAGGCGAAATTCCCCAAAGAACTGAGAAGGTGGGTTA | 637; 573 | N_FIP_f | 2 μM |
| CGGCGGTTCATGGTATCTCTCCAATCCTTGGCTTGTTGC | 688; 751 | N_BIP_f | 2 μM |
| TCACTCTCCTTTGTTGTGTGT | 616 | N_LF_f | 1 μM |
| ATACTTGACATCAAGAGGACCC | 709 | N_LB_f | 1 μM |
