Table 7.
Real-time RT-PCR and the RT-LAMP results from the PPRV animal experiment. The mean values were calculated from 12 animals on different days post infection.
| Blood samples | Ocular swabs | Nasal swabs | ||||
|---|---|---|---|---|---|---|
| Day post infection (dpi) |
RT-PCR [Ct values] mean ± SD (range) |
RT-LAMP [mm:ss] mean ± SD (range) |
RT-PCR [Ct values] mean ± SD (range) |
RT-LAMP [mm:ss] mean ± SD (range) |
RT-PCR [Ct values] mean ± SD (range) |
RT-LAMP [mm:ss] mean ± SD (range) |
| 2 |
35.57*± 1.87 (34.05 to 37.66) |
ND | ND | ND | ND | ND |
| 4 |
28.34 ± 1.57 (26.66 to 31.19) |
13:34 ± 2:45 (10:45 to 19:00) |
32.56 ± 4.71 (21.97 to 39.60) |
15:00 ± 1:57 (12:45 to 16:15) |
30.78 ± 3.95 (26.52 to 36.20) |
16:15 ± 3:03 (11:00 to 19:30) |
| 6 |
23.36 ± 1.11 (22.22 to 25.47) |
09:56 ± 1:27 (8:00 to 12:15) |
26.63 ± 2.84 (20.54 to 30.91) |
13:19 ± 2:23 (10:00 to 18:30) |
25.83 ± 2.60 (23.09 to 30.81) |
12:20 ± 1:21 (10:00 to 14:15) |
| 8 |
24.30 ± 1.35 (23.01 to 27.66) |
10:58 ± 1:27 (9:30 to 13:15) |
22.46 ± 2.14 (19.60 to 26.40) |
12:06 ± 1:29 (10:30 to 14:15) |
21.35 ± 2.17 (17.68 to 24.70) |
10:40 ± 1:04 (9:15 to 12:45) |
| 9** |
24.34 ± 0.68 (23.78 to 25.11) |
9:10 ± 0:17 (9:00 to 9:30) |
22.15 ± 0.41 (21.79 to 22.60) |
8:30 ± 00:54 (7:30 to 9:15) |
23.11 ± 1.93 (21.10 to 24.95) |
8:30 ± 0:26 (8:15 to 9:00) |
ND – not detected.
*
only three animals were tested positive using the real-time RT-PCR.
**
only three animals were still alive and available for testing.