Skip to main content
. 2019 Aug;76:1–12.e5. doi: 10.1016/j.exphem.2019.07.003

Figure 3.

Figure 3

Transcriptional characterization of human EPSCM-derived hematopoietic cells. (A) Schematic of human PSC differentiation from EPSCM into EBs and cell populations sorted for RNA-seq analysis from day 14 and day 20 EBs. For each cell population, a total of 50 cells were sorted per RNA-seq sample. Five RNA-seq samples were processed per cell type. (B) Principal component analysis of the RNA sequencing of the four cell populations (five replicates per population) described in A. (C) Heatmap displaying the mean gene expression of various pluripotent and hematopoietic genes (display as Log2[normalized counts] from the RNA-sequencing analysis. (D) Log2(normalized counts) RNA-seq gene expression of HOXA and HOXB cluster genes within RNA-seq data sets from A. Data are mean ± standard deviation from five samples per cell type. (E and F) Bar graphs displaying p values from Enrichr analysis [39] for the top 250 differentially expressed genes between D14EMP and D14HC samples within Human Cell Atlas (gray bars) and Reactome 2016 (black bars) annotated gene sets. (D) Enrichment for genes upregulated in D14EMP. (E) Enrichment for genes upregulated in D14HC. (G) Heatmap displaying gene expression of the 15 most uniquely upregulated genes for each cell type within each sample. Gene expression displayed as display as Log2(normalized counts), as in C.