Table 2.
Bacterial strains and plasmids used in this study
| Bacterial strain | Relevant characteristics* | Source |
|---|---|---|
| Escherichia coli | ||
| DH‐5α | F‐ deoR endA1 gyrA96 hsdR17(rK ‐mK +) recA1 relA1 supE 44 thi‐1 Δ(lacZYA‐argF)U169(φ80lacZΔM15) | Laboratory stock |
| BL21(DE3) | F‐ dcm ompT hsdS(rB ‐mB ‐) gal (λDE3) | Laboratory stock |
| S17‐1 | Tpr Smr recA thi pro hsdR (RP4‐2 Tc::Mu Km::Tn7), λpir | Laboratory stock |
| CL104 | fabG(ts) panD Cmr, Tetr, Kmr | Laboratory stock |
| MG1655 | Wild‐type | Laboratory stock |
| Xanthomonas campestris pv. campestris | ||
| XC1 | Rifr, wild‐type | Qian et al. (2005) |
| FE58 | Rifr, Tcr, rpfF::Tn5 lac, Biosensor for DSF signals | Wang et al. (2004) |
| YH1 | Rifr, ΔfabG3 | This study |
| YH2 | Rifr, Kmr, Xcc YH1/pYYH‐5 | This study |
| XC1(ΔrpfC) | Rifr, XC1ΔrpfC | Zhou et al. (2015) |
| YH3 | Rifr, XC1ΔrpfCΔfabG3 | This study |
| YH4 | Rifr, Kmr, Xcc YH3/pYYH‐5 | This study |
| YH5 | Rifr, Kmr, XC1ΔrpfC/pYYH‐5 | This study |
| YH6 | Rifr, Kmr, Xcc YH1/pYYH‐6 | This study |
| YH7 | Rifr, Kmr, Xcc YH1/pYYH‐7 | This study |
| YH8 | Rifr, Xcc 8004ΔXanB2 | This study |
| YH9 | Rifr, Xcc 8004ΔXanB2ΔfabG3 | This study |
| YH10 | Rifr, Kmr, Xcc YH1/pYYH‐10 | This study |
| Plasmids | ||
| pET‐28b | Kmr, T7 promoter‐based expression vector | Novagen |
| pMD19‐T | Ampr, TA cloning vector | Takara |
| pSRK‐Km | Kmr, broad‐host‐range expression vector containing lac promoter and lacI q, lacZα+ | Khan et al. (2008) |
| pK18mobscaB | Kmr, sacB‐based gene replacement vector | Schafer et al. (1994) |
| pBAD24M | Ampr; pBAD24 NcoI site replaced by NdeI site | Zhu et al. (2013) |
| pHWG | Ampr, EcfabG gene cloned into plasmid pBAD24M | Lab stock |
| pYYH‐1 | Ampr, Xcc fabG3 gene cloned into plasmid pMD19‐T | This study |
| pYYH‐2 | Ampr, Xcc fabG3 gene cloned into plasmid pBAD24M | This study |
| pYYH‐3 | Kmr, Xcc fabG3 cloned into plasmid pET‐28b | This study |
| pYYH‐4 |
Kmr, about 1000 bp Xcc fabG3 deletion DNA fragment inserted into pK18mobscaB between EcoRI/HindIII sites |
This study |
| pYYH‐5 | Kmr, fabG3 cloned into plasmid pSRK‐Km | This study |
| pYYH‐6 | Kmr, XccfabG1 cloned into plasmid pSRK‐Km | This study |
| pYYH‐7 | Kmr, E.coli fabG cloned into plasmid pSRK‐Km | This study |
| pYYH‐8 | Ampr, Xcc fabG1 gene cloned into plasmid pBAD24M | This study |
| pYYH‐9 |
Kmr, about 1000 bp Xcc xanB2 deletion DNA fragment inserted into pK18mobscaB between EcoRI/HindIII sites |
This study |
| pYYH‐10 | Kmr, XccfabG2 cloned into plasmid pSRK‐Km | This study |
*
Rifr, Tc r, Kmr and Ampr indicate resistance to rifampicin, tetracycline, kanamycin and ampicillin, respectively.