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. 2019 Sep 27;20(12):1662–1681. doi: 10.1111/mpp.12868

Figure 6.

Figure 6

Increased resistance to CMV infection in transgenic petunia plants overexpressing LrNAC35. (A) Disease symptoms of wild‐type (WT) and LrNAC35‐overexpressing lines (3‐1, 5‐6 and 12‐3) at 18 days post‐inoculation (dpi) with CMV. The magnified views of symptoms in systemically infected leaves with CMV are indicated as the insets. Four‐leaf‐stage seedlings were used for inoculation. qRT‐PCR (B) and western blot (C) analyses of CMV coat protein (CMV‐CP) transcript and its protein levels in the uppermost leaves of WT and overexpression lines at 8 or 12 dpi with CMV, and the leaf samples at 12 dpi were used for western blots. 26S rRNA and actin were used as a reference gene and protein, respectively. Disease symptoms (D) and relative cell death area (E) of inoculated leaves of WT and LrNAC35‐overexpressing lines (3‐1, 5‐6 and 12‐3) at 6 dpi with CMV. (F) Electrolyte leakage (conductivity) in the inoculated leaves of WT and transgenic lines challenged with CMV at intervals. (G) Klason lignin content in CMV‐inoculated leaves of WT and transgenic lines at different time points. Error bars represent SE of the mean from three biological replicates. Asterisks indicate significant difference as evaluated by Student's t‐test at P < 0.05.