Table 1.

Summary of primer sets used and the number of reads obtained. ITS = Internal transcribed spacer region of rDNA; IGS = Intergenic spacer region of rRNA; mito = mitochondria; *Only the forward read was analyzed.

Primer Pair (Forward/Reverse)	Gene Region	Target Group	Expected size (nt)	# paired reads	Reference
515F/806R	16S	Prokaryotes	292	23 407 998	1,2
Arch349F/806R	16S	Archaea	457	200 676	3
566F/1200R	18S	Protists	>660*	12 220 122	4
Euk-1391F/EukBr-7R	18S	Protists	95–175	6852 490	5
ITS1F/ITS2R	ITS	Fungi	>580	34 221 582	6
APdsb298F_APdsb670R	dsb	Anaplasma	392	1827 983	7
APdsb365F_APdsb715R	dsb	Anaplasma	370	1985 614	7
BT18SF_BT18SR	18S	Babesia	399–449*	863 988	8
FBbslF_FBbslR2	5S-23S IGS	B. burgdorferi	250	391 485	9
FBLBM16_23F3_FBLBM16_23R3	16S-23S IGS	B. miyamotoi/lonestari	295–350	260 668	9
16Splus1_16Sminus1	16S (mito)	Ixodes	450–454	51 862	10

References: ¹Apprill et al. (2015), ²Bergmann et al. (2011), ³Takai and Horikoshi (2000), ⁴Hadziavdic et al. (2014), ⁵Amaral-Zettler et al. (2009), ⁶Schoch et al. (2012); ⁷Keesing et al. (2018); ⁸Georges et al. (2001); ⁹Fredericks et al., in prep and ¹⁰Nadolny et al. (2011).