Table 4.

Two-way ANOSIMs of bacterial and fungal community similarity with B. burgdorferi presence/absence and Spirurida infection status as factors. Borrelia spp. were filtered from the dataset and Bray–Curtis distance matrices were log (x+1) transformed. Significance was determined by comparing the ANOSIM R to the R values obtained from 999 999 random permutations. For negative R values, the P-value was calculated as (100-P) *2.

			B. burgdorferi			Spirurida
Kingdom	Data	Seqs/sample	N	R	P	R	P
Bacteria	Bray–Curtis	10 700	102	0.162	0.012	0.153	0.022
	Weighted Unifrac			0.144	0.039	0.028	0.346
	Unweighted Unifrac			0.042	0.209	−0.12	0.036
Fungi	Bray–Curtis	11 400	101	−0.067	0.072	0.047	0.126