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. 2019 Apr 17;25:101196. doi: 10.1016/j.redox.2019.101196

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

figs1 — Effects of AOPPs on cyclin D1, cyclin D3, CDK4 and CDK6 expression as well as binding of p27kip1 to cyclinD-CDK4/6 complexes in IEC-6 cells. IEC-6 cells were treated with control medium, RSA (50μg/ml), or the indicated concentration of AOPPs for 24h (a), or 200μg/ml of AOPPs for the indicated times (b), or 200μg/ml of AOPPs for 24h (c). (a, b) Representative Western blotting showing the expression of cyclin D1, cyclin D3, CDK4 and CDK6 in IEC-6 cells after AOPPs treatment. GAPDH was used to verify equivalent loading. (c) Results of co-immunoprecipitation analysis showed that AOPPs treatment did not affect the binding of p27kip1 to cyclinD-CDK4/6 complexes in IEC-6 cells.