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. 2019 Apr 17;25:101196. doi: 10.1016/j.redox.2019.101196

Fig. 3.

Fig. 3

AOPPs-enhanced p27kip1 expression in IEC-6 cells. IEC-6 cells were treated with control medium, RSA (50 μg/ml), or 200 μg/ml of AOPPs for 24 h (a, f), or the indicated concentration of AOPPs for 24 h (b, d) or 200 μg/ml of AOPPs for the indicated times (c, e). (a) Immunofluorescence staining for p27kip1 revealed that AOPPs stimulation enhanced the expression of p27kip1 in IEC-6 cells. Scale bar, 20 μm. (b, c) Representative Western blotting and bar graphs depicting densitometry quantification and showing augmentation of p27kip1 in IEC-6 cells after AOPPs treatment. (d, e) mRNA expression of p27kip1 was determined by qPCR. Bar graphs showing qPCR results revealed that AOPPs treatment increased p27kip1 gene expression in a dose- and time-dependent manner. Relative protein and mRNA levels were normalized with GAPDH, respectively. (f) Results of co-immunoprecipitation analysis revealed that AOPPs treatment enhanced the binding of p27kip1 to cyclin E-CDK2 complexes in IEC-6 cells. Data are presented as mean ± SD from three independent experiments; error bars indicate SD. *P < 0.05 and **P < 0.01 versus control.