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Reduction of H2O2 -induced apoptosis by expression of mito-HIF-1α. A. Transfected HeLa cell viability was assessed after H2O2 treatment for 8 h using the CCK-8 assay. B, C. Flow cytometric analysis of apoptosis (annexin V/PI; B) and caspase activity (TF5-VAD-FMK; C) after H2O2 treatment of transfected HeLa cells for 24 h. Data are the means ± SEM (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, not significant.
