Fig. 3.

Effects of 0.95 ATA O₂ on superoxide production in cSC cells in rat brain slices (300–400 μm thick) as measured by increased dihydroethidium (2.5 μM DHE) fluorescence over time. A) the rate of superoxide production significantly increased from hour 1 (control 0.40 ATA O₂; open histogram) to hour 2 (hyperoxia 0.95 ATA O₂; diagonal striped histogram) by 176% and remained unchanged throughout hour 3 of hyperoxia (black histogram). B-C) a 1:1 ratio of ketone salts (KS; $\beta$-hydroxybutyrate and acetoacetate) were added during the second hour of hyperoxia (hour 3; black histograms). Two and 5 mM KS significantly inhibited superoxide production by 20% (black histograms). Between 48 and 65 cells were analyzed in each experimental run (∼10 cells/brain slice/rat; postnatal age P10-39). Analysis of variance: *P < 0.05; **P < 0.01; ***P < 0.001. Unpublished data from Hinojo et al. [115].