Fig. 4.

Effects of 15-keto PGE₂on STAT3 regulating molecules and PPARγ in MCF10A-ras cells. A. MCF10A-ras cells were treated with 15-keto PGE₂ (20 μM) for 24 h. The expression of JAK2, an upstream kinase of STAT3 and SOCS3, a negative regulator of STAT3, was assessed by Western blot analysis. **p < 0.01, N.S, not significant. B. Nuclear extracts were isolated from MCF10A-ras cells treated with 15-keto PGE₂ (20 μM), and nuclear PPARγ levels were analyzed by Western blot analysis. Lamin B was used as a marker for nuclear protein.