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. 2019 Nov 18;15:134. doi: 10.1186/s13007-019-0524-7

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The modified method can improve the mapping accuracy of the sequencing reads. a, c Alignment of partial rice miR164 and miR167 family precursors (Red letters denote different nucleotides among these miRNA members). The 20-nt tags generated using previous method can be mapped to multiple genes, while the 27-nt tags generated from these genes using the present method can distinguish those differences easily. b, d Tag sequences and frequency obtained from the modified method which contain mature miR164 and miR167 sequence (red letters denote detected nucleotides at mature miRNA end, and the sequence with * indicates this tag derived from miR167 h precursor. Mature miRNA sequences are underlined)