. 2019 Nov 18;15:134. doi: 10.1186/s13007-019-0524-7
© The Author(s) 2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
| Reagents | Volume (μl) |
|---|---|
| Adapter ligated mRNA | 25 |
| RT primer (100 μM) | 2 |
| dNTP mix (10 μM of each) | 2 |
| Total | 29 |