Reagents	Volume (μl)
cDNA	50
10× high fidelity PCR buffer	10
50 mM MgSO4	4
dNTPs (10 mM of each)	2
10 μM 5′ adaptor primer	2
10 μM 3′ adaptor primer	2
Platinum® Taq DNA polymerase high fidelity	0.4
H2O	29.6
Total	100