. 2019 Nov 18;15:134. doi: 10.1186/s13007-019-0524-7
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| Reagents | Volume (μl) |
|---|---|
| PCR product | 22 |
| 10× NEB buffer 3.1 | 3 |
| 10 mM ATP | 3 |
| 10 U/μl EcoP15I | 2 |
| Total | 30 |