FIG. 4.

HFD-fed CKO mice demonstrate accelerated obesity and suppressed WAT peroxisome biogenesis. (A) Body weight of HFD-fed WT and CKO mice was measured every week. (B–E) Subcutaneous and epididymal WAT weight, before and after corrected by body weight. (F) H&E staining, (G) quantification of adipocyte size, and (H) F4/80 immunostaining of WAT are shown. (I) mRNA levels of adiponectin and inflammation-related genes were analyzed. (J) Plasma and (K) WAT LPO levels were measured. (L) 4-HNE immunostaining of WAT and (M) quantification of positive staining area are shown. (N) 8-oxo-dG immunostaining analysis of WAT. (O–Q) Protein and mRNA expressions of peroxisomal proteins are shown. Expression levels of indicated proteins were quantified by densitometry and normalized with β-actin. Figures' original magnification: 200 × ; scale bar: 100 μm (F, H, and L), 50 μm (N). (A–Q) Values represent means ± SE of seven mice per group. *p < 0.05 versus WT HFD mice. 4-HNE, 4-hydroxynonenal; 8-oxo-dG, 8-hydroxydeoxyguanosine; CKO, catalase knock out; H&E, hematoxylin and eosin; LPO, lipid peroxides; WT, wild-type. Color images are available online.