Figure 1.

Neurogenic Factors Reprogram Astrocytes into Neurons after Traumatic Brain Injury

(A and B) Scheme of the AAV-FLEx constructs (A) and experimental design (B).

(C) Photomicrographs showing an overview with GFP⁺ cells at 24 dpi of AAV encoding for GFP, GFP/Ngn2/Nurr1, and GFP/Ascl1/Nurr1.

(D–I) Photomicrographs showing GFP⁺/NeuN⁺ neurons (full arrowheads) and GFP⁺/GFAP⁺ astrocytes (empty arrowheads) at 24 (D) and 72 (G) dpi of GFP, Ngn2/Nurr1, and Ascl1/Nurr1. Example of Z-projection (E and H) of GFP/Ngn2/Nurr1 neurons (dashed square) used for the co-localization analysis (F and I). n = 3, 4, and 4 for GFP, Ngn2/Nurr1, and Ascl1/Nurr1, respectively.

Data are shown as median ± interquartile range (IQR). Student’s t test. ^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗∗p ≤ 0.0001. AAV, adeno-associated virus; dpi, days post-injection. Scale bars: 100 μm (C, left); 50 μm (C, right); 20 μm (D and G).

See also Figures S1 and S2.