REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-GFAP	Dako	Cat. #Z0334; RRID: AB_10013382
Mouse IgG1 anti-NeuN	Merk/Millipore	Cat. #MAB377; RRID: AB_2298772
Rabbit anti-CUX1	Santa Cruz	Cat. #Sc-13024; RRID: AB_2261231
Mouse anti-SATB2	Abcam	Cat. #Ab51502; RRID: AB_882455
Guinea pig anti-SATB2	SYSY	Cat. #327 004; RRID: AB_2620070
Rat anti-CTIP2	Abcam	Cat. #Ab18465; RRID: AB_2064130
Chicken anti-GFP	Aves Labs	Cat. #GFP-1020; RRID: AB_10000240
Rat anti-CD45	BD/Bioscience	Cat. #550539; RRID: AB_2174426
Rabbit anti-Iba1	Wako	Cat. #019-19741; RRID: AB_839504
Guinea pig anti-SOX9	Lab. of Palle Serup	N/A
Rabbit anti-NeuN	Abcam	Cat. #Ab177487; RRID: AB_2532109
Mouse anti-BRN2	Santa Cruz	Cat. sc-393324; RRID: AB_2737347
Rabbit anti-TBR1	Abcam	Cat. ab31940; RRID: AB_2200219
Rabbit anti-FOXP2	Abcam	Cat. ab16046; RRID: AB_2107107
Rabbit anti-TLE4	Santa Cruz	Cat. sc-9125; RRID: AB_793141
Bacterial and Virus Strains
Stable Competent E. coli (High Efficiency)	NEB	Cat.N: C3040
rAAV2/5 CAG-FLEx-EGFP	SFB 870 Viral Vector Facility	https://www.sfb870.mcn.uni-muenchen.de/projects/subcluster-z/z04/index.html
rAAV2/5 CAG-FLEx-Ngn2	SFB 870 Viral Vector Facility	https://www.sfb870.mcn.uni-muenchen.de/projects/subcluster-z/z04/index.html
rAAV2/5 CAG-FLEx-Nurr1	SFB 870 Viral Vector Facility	https://www.sfb870.mcn.uni-muenchen.de/projects/subcluster-z/z04/index.html
rAAV2/5 CAG-FLEx-Ascl1	SFB 870 Viral Vector Facility	https://www.sfb870.mcn.uni-muenchen.de/projects/subcluster-z/z04/index.html
rAAV2/1 hSyn-FLEx-pmScarletI-P2A-GCaMP6s	SFB 870 Viral Vector Facility	https://www.sfb870.mcn.uni-muenchen.de/projects/subcluster-z/z04/index.html
rAAV2/1 hSyn-pmScarletH-P2A-GCaMP6s	SFB 870 Viral Vector Facility	https://www.sfb870.mcn.uni-muenchen.de/projects/subcluster-z/z04/index.html
Chemicals, Peptides, and Recombinant Proteins
OptiPrep Density Gradient Medium	Sigma	Cat. #D1556
DAPI	Sigma	Cat. #28718-90-3
SYBR Green Real-Time PCR Master Mixes	Thermo Fisher Scientific	Cat. #4385612
Fluorogold dye (Hydroxystilbamidine)	Sigma	Cat. #223769-64-0
EdU (5-ethynyl-2′-deoxyuridine)	Invitrogen	Cat. #E10187
Click-iT EdU Alexa Fluor 647 Imaging Kit	Invitrogen	Cat. #C10340
Experimental Models: Cell Lines
293T	ATCC	CRL-3216; RRID: CVCL_0063
Experimental Models: Organisms/Strains
Mouse: B6.Cg-Tg(Gfap-cre)77.6Mvs/2J	The Jackson Laboratory	Stock N: 024098; RRID: IMSR_JAX:024098
Mouse: C57BL/6J	The Jackson Laboratory	Stock N: 000664; RRID: IMSR_JAX:000664
Oligonucleotides
SV40 Forward primer (5′AGCAATAGCATCA CAAATTTCACAA3′)	Laboratory of Eduard Ayuso (D’Costa et al., 2016)	N/A
SV40 reverse primer (5′CCAGACATGATAAGA TACATTGATGAGTT3′)	Laboratory of Eduard Ayuso (D’Costa et al., 2016)	N/A
GFP Forward primer (5′GATGTTGTGGC GGATCTTGA3′)	Laboratory of Eduard Ayuso (D’Costa et al., 2016)	N/A
GFP reverse primer (5′CAACAGCCACAACG TCTATATCATG3′)	Laboratory of Eduard Ayuso (D’Costa et al., 2016)	N/A
Recombinant DNA
Plasmid: pAAV-CAG-FLEx-EGFP	Laboratory of Malin Parmar (Torper et al., 2015)	N/A
Plasmid: pAAV-hSyn-FLEx-pmScarletI-P2A-GCaMP6	Laboratory of Tobias Rose	N/A
Plasmid: pAAV-hSyn-pmScarletH-P2A-GCaMP6s	Laboratory of Tobias Rose	N/A
Plasmid: pAAV-CAG-FLEx-Ngn2	Laboratory of Malin Parmar (Torper et al., 2015)	N/A
Plasmid: pAAV-CAG-FLEx-Nurr1	Laboratory of Malin Parmar (Torper et al., 2015)	N/A
Plasmid: pAAV-CAG-FLEx-Ascl1	Laboratory of Malin Parmar (Torper et al., 2015)	N/A
Plasmid: pHelper	Cell Biolabs	Part N: 340202
Plasmid: pAAV-RC5	Cell Biolabs	Cat.N: VPK-425
Plasmid: LV- PRSVLTR-psi-CAG-GFP-WPRE-LTRSIN	Laboratory of Magdalena Götz (Buffo et al., 2008)	N/A
Plasmid: RV- CAG-Ngn2-IRES-Dsred	Laboratory of Magdalena Götz (Heinrich et al., 2011)	N/A
Software and Algorithms
Zen software 2.3 lite	Carl Zeiss	RRID: SCR_013672; https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html
ImageJ	NIH	RRID: SCR_003070; https://imagej.nih.gov/ij/
Photoshop	Adobe	RRID: SCR_014199; https://www.adobe.com/products/photoshop.html
Illustrator	Adobe	RRID: SCR_014199; https://www.adobe.com/products/illustrator.html
GraphPad Prism 7.0	GraphPad Software	RRID: SCR_002798; https://www.graphpad.com
MATLAB	MathWorks	RRID: SCR_001622; https://www.mathworks.com
Other
FPLC	GE Healthcare	ÄKTA prime plus
HiTrap Q FF anion exchange chromatography column	GE Healthcare	Cat. 17505301