Fig. 6.

Transcriptionally active floral genes are methylated in both females and males of Mercurialis annua. (A) Analysis of DNA methylation at the promoters (-P) of MaAGL1, MaSL1, and MaSL2 by as determined by chop-PCR. A fragment of MaSL1 lacking the CCGG site (no CCGG) was used as an internal control. Left panel is a control of undigested DNA (Ud). Ud, Undigested DNA; H, HpaII; M, MspI; L, molecular size markers in base pairs. (B) Analysis of methylation at the promoter and in the gene-body of MaAP3 and MaSL1 as determined by bisulfite sequencing. The percentage of cytosine methylation for each fragment was determined from at least 10 different clones. (This figure is available in colour at JXB online.)