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. 2019 Sep 7;70(21):6113–6125. doi: 10.1093/jxb/erz402

Fig. 1.

Fig. 1.

GAAP1/GAAP3 interacted with MAPR3 in vivo and in vitro. (A) GAAP1/GAAP3 interacted with MAPR3, shown by dual-membrane yeast two-hybrid assay. GAAP1/GAAP3 was used as bait and MAPR3 as prey. Yeast harbouring GAAP1/GAAP3 and MAPR3 showed a positive colour for the reporter lacZ assayed by X-gal staining and strong growth on SD-trp-leu-his-ade selection medium. Meanwhile, yeast harbouring the vector pairs only with GAAP1/GAAP3 or only MAPR3 showed negative results. (B) Flag-GAAP1 interacted with MAPR3-TAP fusion protein in tobacco leaf cells assayed by Co-IP. (C) Pull-down assay was used to detect the association of GST–GAAP1 fusion protein with MAPR3-TAP.