Fig. 8.

MAPR3 interacted with IRE1B. (A) BiFC assay showed that MAPR3 interacted with IRE1B in tobacco leaf cells. YFP fluorescence was observed when nYFP–IRE1B and MAPR3–cYFP were co-expressed, whilst only the background signal of chlorophyll was observed when nYFP–IRE1B and cYFP or MAPR3–cYFP and nYFP were co-expressed. Arrows indicate punctate or granule signals, and arrowheads point to the mesh or endosome-like signals. (B) The association of the GST–IRE1B fusion protein with MAPR3-TAP was detected by GST pull-down assay.