Fig. 5.

Effects of aerated and stagnant, deoxygenated conditions on cell viability in the root tips of Zea mays subsp. mays inbred line Mi29, introgression line IL-AE91, and Z. nicaraguensis. Plants were grown under aerated conditions for 25–28 d and were then transferred to stagnant, deoxygenated conditions. Cell viability is indicated by the reduction of 2,3,5-triphenyltetrazolium chloride (TTC). Measurements were taken on 4th nodal roots, which were ~100 mm long at the time of transfer. Data are means (±SD), n=3. Different letters indicate significant differences between means across all time-points as determined using one-way ANOVA and Tukey’s test for multiple comparisons (P<0.05).