Fig. 4.
Subcellular localization of DEK40 and expression pattern of Dek40. (A) Fluorescence signals of transiently expressed DEK40–GFP in maize protoplasts. Mitochondria are marked by Mito Tracker (red). Scale bars=10 µm. (B) Subcellular localization of DEK40 protein in the Arabidopsis marker line of mitochondria. The T1 transgenic line roots were used for imaging by confocal microscopy. The fluorescence signals of expressed DEK40–RFP in the Arabidopsis marker line are shown in red. The Arabidopsis marker line (MT-GK) has a specially expressed GFP in mitochondria, which is marked as Mito-GFP. Scale bars=25 µm. (C) The expression profiles of Dek40 in a variety of organs and maize kernels at different developmental stages. Leaf1, juvenile leaves; Leaf2, mature leaves; DAP, days after pollination. The RNA levels were normalized to that of the maize ZmActin gene (GRMZM2G126010). Values are means of three technical replicates and three biological repeats. Error bars represent the SD.