Fig. 2.

The interaction of TAPBPR with GFP-A2 occurs in a glycosylation independent manner. (A) TAPBPR and tapasin were immunoprecipitated from IFN-γ treated HeLa-M cells expressing the panel of GFP-A2 molecules. Non-transduced cells were included as a negative control. Western blot analysis was performed for GFP, TAPBPR, tapasin, UGT1 or calnexin (CNX) on immunoprecipitates and lysates as indicated. Additional repeats are shown in supplementary Fig. 1. (B) Surface expression of GFP-A2 detected using the conformational specific mAb BB7.2 on IFN-γ treated HeLa-M cells (TAPBPR competent) and IFN-γ treated HeLa-M cells with TAPBPR knocked out (TAPBPR deficient) expressing GFP-A2^WT (red line), GFP-A2^N86Q (blue line) and GFP-A2^S88R (green line). Staining of non-transduced HeLa-M cell with BB7.2 is included as a control (black line). Only GFP positive cells were included in the analysis using BB7.2. The GFP levels on cells gated for GFP is shown in the lower panel. The results are representative of three independent experiments. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)