Fig. 5.

TAPBPR binds to non-glycosylated HLA-A*68:02 and HLA-B*27:05. (A) Surface expression of untagged HLA-A*68:02 and HLA-B*27:05, detected with W6/32, on HeLa-M^ABC−KO cells expressing WT (red line), N86Q (blue line) and S88R (green line) HLA variants in the absence of IFN-γ treatment. Staining of non-transduced HeLa-M^ABC-KO cells with W6/32 is included as a control (black line). (B) TAPBPR and tapasin were immunoprecipitated from IFN-γ treated HeLa-M^ABC−KO cells expressing the panel of HLA-A*68:02 and –B*27:05 molecules. Non-transduced cells were included as a negative control. Western blot analysis was performed for the HLA heavy chains, TAPBPR, tapasin, or calnexin (CNX) on immunoprecipitates and lysates as indicated. The results are representative of three independent experiments. Addition repeats are shown in supplementary Fig. 3. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)