Figure 1.

Shows the steps by which an investigator-generated algorithm calculates CC FV. All image slabs were generated by automated segmentation from Angioplex OCTA software on a Zeiss Cirrus HD-OCT Model 5000 device. (A) This panel shows the choriocapillaris slab before and after image standardization via brightness histogram contrast stretching. (B) This panel shows the SCP slab before and after binarization. (C) The final images in panels A and B are overlaid to remove projection artifacts and generate the first image in panel C. The choriocapillaris image then undergoes alignment and cropping, followed by FV quantification using the pixel intensity of the avascular slab as a threshold for measurement. All pixels with intensities at or below the defined threshold are highlighted in red and quantified as shown in panel C. It is important to note that binarization and projection artifact removal do not visually remove shadows generated on the choriocapillaris by the overlying SCP.