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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Curr Protoc Protein Sci. 2019 Dec;98(1):e99. doi: 10.1002/cpps.99

Figure 5.

Figure 5.

Visualization of IR-labelled proteins captured to antibody spots. A) A single antibody was spotted and signal is localized to a circle, indicating capture of IR-labelled protein to its antibody spot. B) Two antibodies were spotted side-by side and signal is only present in one circle, indicating specific capture of the IR-labelled protein by its antibody. C) Two antibodies were spotted side-by-side and protein signal is observed on both spots, indicating non-specific capture of IR-labelled protein.