Skip to main content
. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Oncogene. 2019 Jul 8;38(30):5839–5859. doi: 10.1038/s41388-019-0844-0

Figure 3: SEPT9 overexpression induces MMP upregulation and promotes secretion of proteases into the cell media.

Figure 3:

A. Representative images of protease arrays incubated with cell culture supernatants from MDA-MB-231_SEPT9_v3 cells (Top) and MDA-MB-231 cells transfected with SEPT9 siRNA (Bottom). B. Quantification of MMP3 and MMP13 signals that were most suppressed in conditioned media from MDA-MB-231 transfected with SEPT9 siRNA (** p<0.005) is shown in A. Error bars indicate SEM. Abbreviations: MMP3 - matrix metalloproteinase-3 (Stromelysin-1); MMP13 - matrix metalloproteinase-13 (Collagenase 3). C. Bar graphs depicting the quantification of MMP1, MMP3 and MMP13 in MCF7, MDA-MB-231 and T47D cells over expressing GFP-SEPT9_v3 relative their parental control lines (* p<0.005; ** p<0.001) are shown. Error bars indicate SEM. D. Violin plots depicting the expression levels of MMP9, MMP15, MMP24, and MMP25 in TCGA breast cancer provisional cases expressing SEPT9 mRNA 1.5 folds higher than the mean (n=210) or 1.5 folds lower than the mean (n=883).

(**** p<0.0001; *** p<0.0003; ** p<0.0035).