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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Oncogene. 2019 Jul 8;38(30):5839–5859. doi: 10.1038/s41388-019-0844-0

Figure 5: SEPT9 localizes to Focal Adhesions, gelatin degradation sites, and MMP3.

Figure 5:

A-B. Representative images depicting F-actin (red), SEPT9 (yellow), and vinculin (green) staining of MDA-MB-231 cells. SEPT9 filaments partly colocalize with F-actin filaments (arrow points at FAs extending from SEPT9 filaments). Lower panels in A show enlarged views of the white-boxed regions. C. Representative images depicting matrix (grey), SEPT9 (red), and vinculin (green) staining. Matrix degradation pattern colocalizes with vinculin. MDA-MB-231 cells were seeded on Alexa Fluor 405–labeled gelatin. D. Representative images depicting filamentous MMP3 signals associated with F-actin filaments emerging from the cell center towards focal adhesions (vinculin staining, green). Arrows point at MMP3 foci localized at sites of FAs. Lower panels show enlarged views of the white-boxed region. Bar=10μm. Cells were seeded on non-fluorescent gelatin.