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. Author manuscript; available in PMC: 2020 Jul 1.

Published in final edited form as: Br J Haematol. 2019 Feb 27;186(2):329–333. doi: 10.1111/bjh.15807

Figure 2. — (A) Mortality in B6 mice following haemin (210 μmol/kg) challenge in the presence (P-Sel^PLT−/−) or absence (P-Sel^EC−/−) of P-selectin on vascular endothelium. Haem induces (B) severe hypoxaemia, (C) lung damage and (D) pulmonary oedema in presence of endothelial P-selectin (P-Sel^PLT−/−) in B6 mice. (E) Survival of SS^P-SelEC+/+ and SS^{P-SelEC−/−} chimeric mice following infusion of haem (70 μmol/kg) (n=7; Mantel-Cox test; p<0.05). (F) Lung function, determined by SpO₂ in SS^P-SelEC+/+ mice, and SS^{P-SelEC−/−} mice that died (SS^{P-SelEC−/−} -D) or survived (SS^{P-SelEC−/−} -S) following haem infusion (n=3; p<0.001). (G) Representative haematoxylin and eosin stained lung sections showing histopathology of lung following haem challenge in the indicated mice group. Note both SS^P-SelEC+/+ and SS^{P-SelEC−/−} -D mice had severe lung congestion (H) Gravimetric assessment of lung oedema (n = 3–6). Statistical analysis was performed using one-way ANOVA (*P < 0.01).

Figure 2. — (A) Mortality in B6 mice following haemin (210 μmol/kg) challenge in the presence (P-Sel^PLT−/−) or absence (P-Sel^EC−/−) of P-selectin on vascular endothelium. Haem induces (B) severe hypoxaemia, (C) lung damage and (D) pulmonary oedema in presence of endothelial P-selectin (P-Sel^PLT−/−) in B6 mice. (E) Survival of SS^P-SelEC+/+ and SS^{P-SelEC−/−} chimeric mice following infusion of haem (70 μmol/kg) (n=7; Mantel-Cox test; p<0.05). (F) Lung function, determined by SpO₂ in SS^P-SelEC+/+ mice, and SS^{P-SelEC−/−} mice that died (SS^{P-SelEC−/−} -D) or survived (SS^{P-SelEC−/−} -S) following haem infusion (n=3; p<0.001). (G) Representative haematoxylin and eosin stained lung sections showing histopathology of lung following haem challenge in the indicated mice group. Note both SS^P-SelEC+/+ and SS^{P-SelEC−/−} -D mice had severe lung congestion (H) Gravimetric assessment of lung oedema (n = 3–6). Statistical analysis was performed using one-way ANOVA (*P < 0.01).