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. 2019 Aug 14;56(11):750–757. doi: 10.1136/jmedgenet-2019-106031

Figure 2.

Figure 2

The morphology of CFAP65 mutant spermatozoa in two affected individuals. (A) H&E staining analysis of the morphology of spermatozoa from NC and CFAP65 mutant individuals (F1: II-2 and F2: II-2). All CFAP65 mutant spermatozoa have much shorter or irregular flagella than those of NC. Scale bars represent 10 µm. (B) Scanning electron microscopy of the spermatozoa of NC and CFAP65 mutant individuals (F1: II-2 and F2: II-2). Compared with spermatozoa with normal morphology in NC, malformed heads, such as amorphous, tapered or small heads, were often observed in F1: II-2 and F2: II-2 (I–VI). Most spermatozoa from both affected individuals exhibited flagella that are short, coiled, bent or had irregular width (I–VI). Scale bars represent 1 µm. NC, normal control.