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. 2019 Nov 18;14(11):e0225343. doi: 10.1371/journal.pone.0225343

Table 1. Identification results (as log scores) regarding V. anguillarum incubation time and temperature for strains cultured on Tryptone soy agar (TSA) containing 0.5% NaCl and supplemented with 1.5% NaCl.

Strain ID 15°C 22°C 37°C
48h 72h 7d 48h 72h 7d 48h 72h 7d
1 305/C/02 2.102 2.182 2.100 2.167 2.075 1.868 2.213 2.133 1.392
2 68/05 2.136 2.187 1.925 2.047 2.187 1.821 2.077 2.006 1.349
3 82/05 2.062 2.257 1.996 2.203 2.180 1.879 2.149 2.161 1.506
4 372/05 2.118 2.243 2.064 2.138 2.234 1.852 2.237 2.013 1.456
5 31/06 2.058 2.144 2.079 2.201 2.228 1.875 2.195 2.019 1.614
6 136/C/06 2.206 2.265 2.034 2.219 2.256 1.882 2.283 2.228 1.373
7 77/09 2.192 2.208 1.959 2.198 2.226 1.912 2.205 2.145 1.262
8 135/09 2.157 2.220 2.010 2.143 2.219 2.013 2.228 2.140 1.379
9 158/B/09 2.153 2.248 2.227 2.251 2.135 1.934 2.162 2.148 1.313
10 84/10 2.183 2.371 2.092 2.290 2.218 1.881 2.149 2.234 1.765
11 246/D/10 2.175 1.724 2.064 2.203 2.210 2.047 2.212 2.131 1.715
12 65/A/11 2.145 2.183 2.148 2.230 2.214 2.006 2.207 2.199 1.819
13 83/A/11 2.141 2.181 2.080 2.196 2.137 1.999 2.236 2.237 1.461
14 104/B/12 2.152 2.291 2.003 2.236 2.148 1.999 2.182 2.206 1.446
15 117/13 2.272 2.271 2.034 2.238 2.165 1.992 2.316 2.146 1.450
16 189/13 2.128 2.229 2.041 2.330 2.136 1.904 2.210 2.167 1.422
17 440/14 2.243 2.194 2.027 2.249 2.168 1.860 2.124 2.196 1.364
18 449/A/14 2.199 2.254 2.101 2.288 2.073 1.972 2.185 2.185 1.288
19 4/B/15 2.121 2.247 2.069 2.289 2.124 1.951 2.266 2.162 1.384
20 73/15 2.142 2.224 2.054 2.339 2.168 2.006 2.132 2.152 1.240
21 121/15 2.152 2.240 2.036 2.299 2.201 1.962 2.213 2.210 1.205
22 130/F/15 2.193 2.134 2.045 2.275 2.090 1.921 2.192 2.122 1.523
23 188/15 2.115 2.083 2.044 2.255 2.091 1.926 2.097 2.088 0.882
24 199/A/16 2.158 2.155 2.134 2.197 2.102 1.891 2.158 2.211 1.361
25 167/C/17 2.173 2.158 2.031 2.227 2.235 1.943 2.158 2.210 1.215
26 141/17 2.203 2.126 2.102 2.268 2.237 1.936 2.141 2.178 1.411
27 13/18 2.056 2.151 2.113 2.301 2.172 1.953 2.249 2.211 1.484

Strains were incubated at 15°C, 22°C, and 37°C and identified at several time-points (48h, 72h and 7d). The samples were applied on the target plate by the on-target extraction method. Scores presented are the average of up to five measurements. Highlighted fields show probable species level identification scores, while highlighted fields with boldface type log scores show highly probable species level identification.