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. 2019 Nov 18;14(11):e0225343. doi: 10.1371/journal.pone.0225343

Table 2. Identification results (as log scores) regarding V. anguillarum incubation time and temperature for strains cultured on Tryptone soy agar (TSA) containing 0.5% NaCl.

Strain ID 15°C 22°C 37°C
48h 72h 7d 48h 72h 7d 48h 72h 7d
1 305/C/02 2.193 2.134 1.928 2.177 2.218 1.747 2.197 2.079 0.814
2 68/05 2.145 2.148 1.988 2.078 2.194 1.744 2.166 2.033 1.319
3 82/05 2.170 2.191 2.056 2.161 2.189 1.818 2.148 1.967 1.558
4 372/05 2.169 2.152 1.965 2.063 2.175 1.794 2.138 2.040 1.527
5 31/06 2.237 2.186 1.972 2.122 2.139 1.893 2.243 1.941 1.260
6 136/C/06 2.241 2.191 1.939 2.195 2.086 1.886 2.159 2.052 1.427
7 77/09 2.202 2.175 1.994 2.107 2.164 1.648 2.126 2.038 1.517
8 135/09 2.263 2.102 2.000 2.074 2.095 1.846 2.164 2.027 0.859
9 158/B/09 2.240 2.173 2.052 2.064 2.197 1.998 2.121 2.068 1.208
10 84/10 2.169 2.184 1.875 2.255 2.265 1.691 2.169 1.985 1.971
11 246/D/10 2.213 2.172 1.977 2.093 2.229 1.867 2.222 2.042 1.610
12 65/A/11 2.160 2.234 2.024 2.107 2.185 1.965 2.274 2.089 2.136
13 83/A/11 2.069 2.137 2.039 2.176 2.198 1.717 2.040 2.293 1.495
14 104/B/12 2.106 2.213 1.980 2.185 2.138 1.892 2.071 2.152 1.397
15 117/13 2.195 2,140 2.045 2.084 2.223 1.767 2.026 2.052 1.535
16 189/13 2.052 2.100 2.023 2.096 2.134 1.871 2.240 2.090 0.927
17 440/14 2.064 2.224 1.922 2.168 2.241 1.803 2.072 1.981 1.306
18 449/A/14 2.004 2.118 1.971 2.107 2.242 1.812 2.096 2.079 1.313
19 4/B/15 2.081 2.217 2.043 2.084 2.222 1.900 2.136 2.118 1.498
20 73/15 2.038 2.089 2.046 2.107 2.110 1.764 2.220 2.323 1.365
21 121/15 2.057 2.194 2.008 2.136 2.170 1.694 2.179 2.081 1.281
22 130/F/15 2.133 2.183 2.115 2.194 2.157 1.727 2.173 2.078 1.460
23188/15 2.083 2.084 1.806 2.098 2.121 1.537 2.049 1.947 1.240
24 199/A/16 2.130 2.135 2.032 2.131 2.245 1.795 2.146 2.098 1.454
25 167/C/17 2.222 2.170 1.963 2.072 2.162 1.836 2.079 2.126 1.326
26 141/17 2.281 2.164 2.002 2.166 2.165 1.778 1.827 2.040 1.261
27 13/18 2.206 2.125 2.007 2.164 2.259 1.890 2.169 1.888 1.124

Strains were incubated at 15°C, 22°C, and 37°C and identified at several time-points (48h, 72h and 7d). The samples were applied on the target plate by the on-target extraction method. Scores presented are the average of up to five measurements. Highlighted fields show highly probable species level identification scores, while the highlighted field with boldface type log score shows highly probable species level identification.