Table 3. Influence of culture conditions (temperature, duration of incubation, culture media (supplementation with 1.5% NaCl)) on the quality of MALDI-TOF mass spectra obtained for 27 strains of V. anguillarum.

Culture conditions		ID to genus level only (%)	Observed ID to species level (%) / prediction*	NI (%)
TSA supplemented with 1.5% NaCl	15°C; 48h		100 / 100
	15°C; 72h	3.70	96.30 / 99.98
	15°C; 7d	11.11	88.89 / 47.83
	22°C; 48h		100 / 99.94
	22°C; 72h		100 / 99.50
	22°C; 7d	85.19	14.81 / 16.11
	37°C; 48h		100 / 99.56
	37°C; 72h		100 / 96.59
	37°C; 7d	11.11	0 / 2.68	88.89
TSA containing 0.5% NaCl	15°C; 48h		100 / 99.99
	15°C; 72h		100 / 99.89
	15°C; 7d	48.15	51.85 / 47.83
	22°C; 48h		100 / 99.59
	22°C; 72h		100 / 96.81
	22°C; 7d	85.19	0 / 2.87	14.81
	37°C; 48	3.70	96.30 / 97.22
	37°C; 72h	22.22	77.78 / 81.31
	37°C; 7d	3.70	3.70 / 0.42	92.60

Percentage of identification to genus/species level was calculated for a total number of strains in relation to culture conditions. Log scores <1.700 were considered unreliable and therefore strains with scores <1.700 for particular measurements/conditions were considered as not identifiable (NI).

* Predictions of probabilities for a positive species identification based on a generalized linear logistic regression model where positive identification was taken as a log score > = 2.000.