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. 2019 Nov 6;13(11):e0007346. doi: 10.1371/journal.pntd.0007346

Fig 3. All Aag2-derived clonal cell lines have a functional RNAi pathway.

Fig 3

C6/36 cells, the parental Aag2 cell line and its derived clonal cell lines were transiently transfected with plasmids constitutively expressing firefly luciferase and Renilla luciferase (transfection control) in the presence of dsRNA directed against GFP (dsGFP) or firefly luciferase (dsLuc). Mean Renilla-normalised firefly luciferase (FFluc) expression is expressed relative to the dsGFP negative control. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant (one-tailed Student’s t test). Error bars represent standard deviation. # Aag2-AF5 cell line used for CRISPR gene editing [60,61] is highlighted in orange. PCLV-low clones Aag2-AF10 and Aag2-AF12 are highlighted in green; parental Aag2 cells and C6/36 cells (negative control) are shown in purple.