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. 2019 Nov 6;13(11):e0007346. doi: 10.1371/journal.pntd.0007346

Fig 5. Transfection efficiency of clone Aag2-AF5 relative to parental Aag2 cells.

Fig 5

(A) Cells were imaged at 10X magnification 48 h after transient transfection with a constitutively active GFP expression vector (pIEx-EGFP). (B) Quantification of transfection efficiency; differences are non-significant. (C) Cells were transiently transfected with a constitutively active firefly luciferase reporter plasmid (pIEx-luc) and luciferase activity was measured four days later. * P < 0.05 (two-tailed Student’s t test). RLU, relative light units. All error bars represent standard deviation. # Aag2-AF5 cell line used for CRISPR gene editing [60,61].