Figure 2.

Hypothalamic expression of genes involved in appetite regulation. Expression of 10 000 genes and open reading frames was determined by microarray analysis using pooled RNA samples from n = 6 rats exposed to control diet (18% casein) in utero or n = 6 low protein diet (9% casein) for the first week of fetal life (0–7 days gestation). Data show relative expression in low protein diet exposed offspring at 12 weeks of age. Microarray analysis showed that only 19 genes were up‐regulated and 24 genes were down‐regulated by intrauterine undernutrition (taking a twofold change in expression as the chief criterion for identification as up‐ or down‐regulated). Gabra A4, gamma‐aminobutyric acid receptor subunit 4; Alpha‐A, alpha‐adrenergic receptor A1; Gal2R, galanin receptor subtype 2.