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. 2019 Nov 13;6(6):ENEURO.0124-19.2019. doi: 10.1523/ENEURO.0124-19.2019

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Copyright © 2019 Pati et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Dose-dependent effects of acute chemogenetic hM3Dq activation on TBS-induced LTP in hippocampal slices. A, Shown are representative fESPS traces before, 30 min after CNO treatment and 20 min after LTP induction. B, Slices with 30 min of 1 μM CNO bath application or sham-administered control, show robust induction in LTP, with failure to induce LTP at 20 μM CNO treatment. Bath application of 1 μM CNO results in a significant increase in early-phase LTP as compared to sham-treated controls. C, fEPSP slope normalized to pre-LTP baseline shows robust potentiation with bath application of both sham and 1 μM CNO, but not at 20 μM CNO; *p < 0.05 as compared between 1 and 20 μM CNO treatment (linear regression followed by ANCOVA) for time-course analysis. The average fEPSP slope in 5-min time bins is significantly increased in 5- to 10-, 25- to 30-, and 55- to 60-min bins as compared to pre-LTP in aCSF bath (sham; D) as well as with 1 μM CNO bath application (E). The average fEPSP slope in 5-min time bins was significantly increased 5- to 10- and 25- to 30-min bins as compared to pre-LTP with 20 μM CNO bath application (F). The average fEPSP slope was not significantly different in 55- to 60-min bin as compared to pre-LTP with 20 μM CNO bath application (F). Results are expressed as the mean ± SEM; *p < 0.05 as compared between pre-LTP and post-LTP (5–10 min), post-LTP (25–30 min), or post-LTP (55–60 min; one-way ANOVA followed by post hoc Bonferroni multiple comparison). G, The average fEPSP slope in 5-min time bins is significantly increased in 5- to 10 and 25- to 30-min bins as compared to pre-LTP with 1 μM CNO bath application. The average fEPSP slope with 20 μM CNO bath application in 5- to 10- and 25- to 30-min bins was significantly lower compared to both sham-treated control and 1 μM CNO treatment. The average fEPSP slope in the 55- to 60-min bin was significantly lower with both 1 and 20 μM CNO bath application compared to sham-treated control. Results are expressed as the mean ± SEM; *p < 0.05 as compared between sham, 1 μM, and 20 μM CNO treatment for each time bin (one-way ANOVA followed by post hoc Bonferroni multiple comparison).