Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 18;8:e48255. doi: 10.7554/eLife.48255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019, Herring et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 4. — (A) Mouse neuroblastoma cells (Neuro-2a) were cultured and subjected to a subcellular fractionation to isolate the cytosolic, crude mitochondrial, and nuclear fractions. 30 μg of each fraction was analyzed for CLU protein expression using anti-CLU H-330. Fraction purity was analyzed using fraction-specific markers: Gapdh (cytosol), Grp75 (mitochondria), and lamin A (nucleus). (B) Neuro-2a cells were transfected with 200 pM of Exon-3-targeting siRNA or non-specific scramble for 18 hr followed by 72 hr incubation. Whole cell lysates were analyzed for CLU protein expression using anti-CLU H-330. To ensure consistent loading, blots were stripped and re-probed for Hsp60 immunoreactivity. The relative intensity of CLU_45 kDa was normalized to the relative intensity of Hsp60 and is plotted as % change compared to the non-transfected cells. Values represent the mean of two individual experiments, with error bars representing the standard error.