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. 2019 Nov 18;8:e48255. doi: 10.7554/eLife.48255

Figure 6. Mouse CLU_45 kDa is translated from a non-canonical CUG start site in Exon 3.

Figure 6.

(A) Sequential alignment of human and mouse CLU: DNA of Exons 2 and 3. Green sequences represent the Exon 2 translational start site (ATG) in both mouse and human CLU. Blue and yellow sequences represent the Exon 3 translational start site for human CLU (ATG) and the proposed Exon 3 translational start site for mouse CLU_45 kDa (CTG), respectively. (B) Empty vector (pCMV6), Myc-tagged pCMV6-mouse CLU Exon 2–9, or Exon 3–9 constructs were transfected into Neuro-2a cells. Cell lysates and culture media were harvested and probed for CLU immunoreactivity using the anti-Myc antibody. (C) pCMV6-mouse CLU Exon 2–9 or Exon 3–9 constructs were transfected into Neuro-2a cells. Cell lysates and culture media were harvested and treated with endoglycosidases. The resulting samples were analyzed for CLU immunoreactivity using the anti-Myc antibody.