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. 2019 Nov 18;8:e48255. doi: 10.7554/eLife.48255

Figure 8. Human CLU_45 kDa is translated from an AUG in Exon 3 and is present in human primary neurons and astrocytes.

Figure 8.

(A: upper panel) Alignment of human and mouse CLU: amino-acid sequences of Exons 2 and 3. Green represents the Exon 2 start site in both human and mouse CLU (Met), blue represents the Exon 3 start site for human CLU (Met), and yellow represents the Exon 3 start site for mouse CLU (Leu). (A: lower panel) SH-SY5Y cells were transfected with empty vector (pCMV6) or pCMV6-human CLU Exon 3–9 amino-acid 34 variants: 34_ATG (wild-type), 34_CTG (mimics mouse CLU), or 34_CTA (negative control). Cell lysates were probed for CLU immunoreactivity using the anti-V5 antibody. (B) Human primary cortical neurons and astrocytes were cultured and imaged to demonstrate cell type visually. Increasing concentrations of whole cell lysate were probed for CLU_45 kDa immunoreactivity using anti-CLU AF2937.