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. 2019 Nov 18;8:e48255. doi: 10.7554/eLife.48255

Figure 9. Mitochondrial CLU_45 kDa is present in the CLU–/–animal model.

Figure 9.

(A) Total RNA was isolated from WT and CLU–/– cortical tissue. 1.5 µg RNA was reverse transcribed and 50 ng cDNA was analyzed for Exon 2- and Exon 3–4-containing mRNA. To confirm equal loading, β-actin mRNA was amplified using both WT and CLU-/- cDNA. Following amplification, amplicons were run on a 2% agarose gel. (B) 30 µg cortical tissue lysate isolated from WT and CLU–/– mice was analyzed by immunoblotting and probed with anti-CLU M-18 (left panel) or anti-CLU B-5 (right panel) overnight at 4°C. Blots were washed and probed with species-specific HRP-conjugate secondary antibodies.