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. 2019 Sep 29;54(3):1900154. doi: 10.1183/13993003.00154-2019

FIGURE 2.

FIGURE 2

Increased interleukin (IL)-1α and IL-1β levels in the fos-related antigen-2 (Fra-2)-overexpressing (transgenic (TG)) mouse. BALF: bronchoalveolar lavage fluid; AP-1: activator protein-1. a, b) IL-1α and IL-1β protein levels in a) lung homogenate and b) BALF of 20-week-old WT and Fra-2 TG mice. c) Immunohistochemical staining of IL-1α and IL-1β on lung sections of Fra-2 TG and WT mice. B: bronchi, V: vessel; black arrows: bronchial epithelial cells; white arrows: structural and inflammatory cells. Scale bar: 20 µm. d) Schematic representation of the human IL1A and mouse Il1a promoter sequences including AP-1-binding sites. e, f) Electrophoretic mobility shift assay analysis performed on nuclear extracts of e) Fra-2-overexpressing primary human parenchymal fibroblasts or cells transfected with empty control plasmid (–) and f) lung homogenates from WT or Fra-2 TG mice. –NE: negative control of the binding reaction without nuclear extract. For loading and overexpression controls, see supplementary figure S1. **: p<0.01.