Table 1. Number of replicate samples per participant by fecal collection method and day of freezing in the meta-analyses of pooled samples from five studies (Mayo 1-Knight lab, Mayo 1-Mayo lab, Mayo 2, Bangladesh, and Colorado).
| No. of aliquots frozen on: | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Day 0a | Day 4b | |||||||||||
| Study, number of individuals in each study | No-solution | FIT | FOBT/FTA cardsc | 70% ethanol | 95% ethanol | RNAlater | No-solution | FIT | FOBT/FTA cardsc | 70% ethanol | 95% ethanol | RNAlater |
| Mayo 1d, n = 20 [16] | 3 | 0 | 3 | 2 | 0 | 2 | 3 | 0 | 3 | 2 | 0 | 2 |
| Mayo 2, n = 52 [14] | 3 | 2 | 3 | 0 | 2 | 2 | 0 | 2 | 3 | 0 | 2 | 2 |
| Bangladesh, n = 50 [15] | 2 | 2 | 3 | 0 | 2 | 2 | 0 | 2 | 3 | 0 | 2 | 2 |
| Coloradoe, n = 10 [13] | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
a On Day 0, a total of 326 no-solution samples, 204 FIT tubes, 376 FOBT/FTA card samples, 50 70% ethanol samples, 214 95% ethanol samples, and 254 RNAlater samples were analyzed for each sample collection method
b On Day 4, the number of samples analyzed were the same as those described in footnote ‘a’, except a total of 70 no-solution samples were analyzed
c FOBT cards were a triple-slide (3-window) card on day 0 or day 4. Three windows were used per card, and each window was considered a separate aliquot.
d Samples were analyzed at Knight laboratory and Mayo laboratory
e Samples were frozen on Day 7
Abbreviations: FIT, fecal immunochemical test tubes; FOBT, fecal occult blood test cards; FTA, Flinders Technology Associates card