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. 2019 Oct 29;8:e48601. doi: 10.7554/eLife.48601

Figure 1. Mask is required to promote nuclear localisation of Yki in Drosophila follicle cells.

(A) Stage 10 Drosophila egg chamber with endogenously tagged Yki-GFP (green) localised to the nucleus of stretch cells (anterior) and cytoplasm of columnar cells (posterior). (A’) Magnification of columnar cells. (B) Stage 11 Drosophila egg chamber with endogenously tagged Yki-GFP (green) localised to the nucleus of stretch cells (anterior) and nucleus of flattening columnar cells caused by growth of the oocyte (posterior). (B’) Magnification of flattening columnar cells. (C) Stage 10 Drosophila egg chamber containing null mutant clones of mask (marked by absence of nuclear RFP, red) display cytoplasmic Yki-GFP. (C’) Yki-GFP single channel. (D) Stage 11 Drosophila egg chamber containing null mutant clones of mask (marked by absence of nuclear RFP, red) display cytoplasmic Yki-GFP. (D’) Yki-GFP single channel. (E) Quantification of nuclear:cytoplasmic ratio of Yki-GFP in (C) n = 7 clones. (F) Quantification of nuclear:cytoplasmic ratio of Yki-GFP in (D) n = 12 clones.

Figure 1.

Figure 1—figure supplement 1. Mask has no intrinsic transcriptional co-activator activity in a GAL4-UAS reporter assay.

Figure 1—figure supplement 1.

Drosophila S2 cells were transfected with a UAS.Luciferase reporter gene and various GAL4 linked constructs, including GAL4 DNA binding domain (DBD) alone, GAL4-DBD-Yki and GAL4-DBD-Mask. No effect of Mask on luciferase reporter activity was measured, while Yki had a very strong transcriptional activity on the UAS.Luciferase reporter gene.