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. 2019 Oct 8;8:e49599. doi: 10.7554/eLife.49599

Table 2. Sequences of MAGIC probes and corresponding target sites.

MAGIC Probe Target Sequence Target site
Human β-actin 5'-GGATAGCACAGCCTGGATAG-3' 507-488
Human myosin heavy chain α (MHCα) 5'-GGCACCAATGTCACGGCTCT-3' 5864-5845
Human myosin light chain 2a (MLC2a) 5’-GGCCTGCTTGGTGGCTGCCA-3’ 66-47
Human NKX2-5, Probe 1 5’-GGCTGCGCTGCTGCTGTTCC-3’ 308-289 
Human NKX2-5, Probe 2 5’-GGACGTGAGTTTCAGCACGC-3’ 766-747
Human NKX2-5, Probe 3 5’-GCGTTATAACCGTAGGGATT-3’ 984-965
Control Probe 5'-GGATCGTGACTAGATCGTCA-3'
Labeled ssRNA for EMSAs 5'-GGATGAGTCACTGCCTAGCC-3'
Unlabeled ssRNA complementary to probe above 5'-GGCTAGGCAGTGACTCATCC-3'
Labeled ssRNA for multiplex spectral FRET 5'-GGAAGTAGCACAGTCCAGAC-3'
Unlabeled ssRNA for affinity purification, strand 1 5'-GAGTCCTTCCACGATAGACC-3'
Unlabeled ssRNA for affinity purification, strand 2 5'-GGTCTATCGTGGAAGGACTC-3'